Goat anti-CHRNB4 Antibody

Item#:

dAP-1398

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-CHRNB4 Antibody
Principle Name	CHRNB4
Official Symbol	CHRNB4
All Name and Symbols	CHRNB4 ; cholinergic receptor; nicotinicN
Accession Number(s)	NP_000741.1
Peptide Sequence	DYRLTWNSSRYEGVN, is from internal region
Human Gene ID(s)	1143
Non-Human GeneID(s)
Important Comments	The immunizing peptide represents part of the first extracellular domain (aa22-236).
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, IHC
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 16000.
Western Blot	Preliminary experiments gave an approx 40kDa band in human testis lysates after 0.2µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 56.4
Immunohistochemistry	Paraffin embedded Human Uterus. Recommended concentration
Reference(s)	Liang Y, Salas R, Marubio L, Bercovich D, De Biasi M, Beaudet AL, Dani JA. Functional polymorphisms in the human beta4 subunit of nicotinic acetylcholine receptors. Neurogenetics. 2005 Feb;6(1)