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Goat anti-Dual oxidase 1 Antibody
Item#:
dAP-1396
Size Price Quantity
50.0 ug $310.00
Goat anti-Dual oxidase 1 Antibody
Principle Name Dual oxidase 1
Official Symbol DUOX1
All Name and Symbols DUOX1 ; dual oxidase 1 ; LNOX1; MGC138840; MGC138841; NOXEF1; THOX1; NADPH thyroid oxidase 1; flavoprotein NADPH oxidase; nicotinamide adenine dinucleotide phosphate oxidase
Accession Number(s) NP_059130.2 ; NP_787954.1
Peptide Sequence EKFQRSCLHQTVQ, is from internal region
Human Gene ID(s) 53905
Non-Human GeneID(s)
Important Comments This antibody is expected to recognise both reported isoforms (NP_059130.2 ; NP_787954.1) which are identical;
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 32000.
Western Blot Preliminary experiments in Human Placenta and Thyroid Gland lysates gave no specific signal but low background (at antibody concentration up to 0.5µg/ml). We would appreciate any feedback from people in the field - have any results been rep
Immunohistochemistry In paraffin embedded Human Thyroid shows staining of membranes in epithelial cells of the gland. Recommended concentration, 4-6µg/ml.
Reference(s) Harper RW, Xu C, Eiserich JP, Chen Y, Kao CY, Thai P, Setiadi H, Wu R. Differential regulation of dual NADPH oxidases/peroxidases, Duox1 and Duox2, by Th1 and Th2 cytokines in respiratory tract epithelium. FEBS Lett. 2005 Aug 29;579(21)

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