Goat anti-NOVA1 Antibody |
| Principle Name | NOVA1 |
| Official Symbol | NOVA1 |
| All Name and Symbols | NOVA1; neuro-oncological ventral antigen 1; Nova-1; paraneoplastic Ri antigen; ventral neuron-specific protein 1 |
| Accession Number(s) | NP_002506.2 ; NP_006480.2 ; NP_006482.1 |
| Peptide Sequence | REMPQNVAKTEPVS, is from internal region |
| Human Gene ID(s) | 4857 |
| Non-Human GeneID(s) | |
| Important Comments | This antibody is expected to recognise all three reported isoforms (NP_002506.2 ; NP_006480.2 ; NP_006482.1) |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 64000. |
| Western Blot | In transfected HEK293 transiently expressing NOVA1 a band of approx. 52kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration |
| Immunohistochemistry | In paraffin embedded Human Cerebral Cortex shows staining of selected nuclei. Recommended concentration, 2-4µg/ml. |
| Reference(s) | Jelen N, Ule J, Zivin M, Darnell RB. Evolution of Nova-dependent splicing regulation in the brain. PLoS Genet. 2007 Oct 12;3(10) |
