Goat anti-CES1 Antibody |
| Principle Name | CES1 |
| Official Symbol | CES1 |
| All Name and Symbols | CES1; carboxylesterase 1 (monocyte/macrophage serine esterase 1); ACAT; CEH; CES2; HMSE; HMSE1; MGC117365; PCE-1; SES1; TGH; acyl coenzyme A |
| Accession Number(s) | NP_001020366.1; NP_001020365.1; NP_001257.4 |
| Peptide Sequence | NTQAAQKLKDKE, is from internal region (near C terminus) |
| Human Gene ID(s) | 1066 |
| Non-Human GeneID(s) | |
| Important Comments | This antibody is expected to recognise all three reported isoforms (NP_001020366.1; NP_001020365.1; NP_001257.4). |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 8000. |
| Western Blot | Approx 55-60kDa band observed in Human Liver and Ileum lysates (calculated MW of 62.6kDa according to NP_001020366.1). In transfected HEK293 transiently expressing CES1 a band of approx. 65kDa is observed. This band is not observed in the n |
| Immunohistochemistry | In paraffin embedded Human Lung shows staining of lymphocytes in the vasculature.. Recommended concentration, 2-3µg/ml. |
| Reference(s) | Steinberg GR, Kemp BE, Watt MJ. Adipocyte triglyceride lipase expression in human obesity. Am J Physiol Endocrinol Metab. 2007 Oct;293(4) |
