Goat anti-DNMT1 Antibody |
| Principle Name | DNMT1 |
| Official Symbol | DNMT1 |
| All Name and Symbols | DNMT1; DNA (cytosine-5-)-methyltransferase 1; CXXC9; DNMT; FLJ16293; MCMT; MGC104992; DNA methyltransferase 1 |
| Accession Number(s) | NP_001370.1 |
| Peptide Sequence | RFESPPKTQPTEDN, is from internal egion |
| Human Gene ID(s) | 1786 |
| Non-Human GeneID(s) | 13433 (mouse); 84350 (rat); |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 32000. |
| Western Blot | Approx 170kDa band observed in lysates of cell line Jurkat (calculated MW of 183kDa according to NP_001370.1). Recommended concentration |
| Immunohistochemistry | In paraffin embedded Human Kidney shows nuclear staining in PCT. Recommended concentration, 3-6µg/ml. |
| Reference(s) | Dion V, Lin Y, Hubert L Jr, Waterland RA, Wilson JH. Dnmt1 deficiency promotes CAG repeat expansion in the mouse germline. Hum Mol Genet. 2008 May 1;17(9) |
