Goat anti-Phorbolin 1 / APOBEC3A Antibody

Item#:

dAP-1351

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-Phorbolin 1 / APOBEC3A Antibody
Principle Name	Phorbolin 1 / APOBEC3A
Official Symbol	APOBEC3A
All Name and Symbols	APOBEC3A; apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3A; ARP3; PHRBN; bK150C2.1; phorbolin 1
Accession Number(s)	NP_663745.1
Peptide Sequence	TSNFNNGIGRHKTY, is from internal region
Human Gene ID(s)	200315
Non-Human GeneID(s)
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, IHC
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 8000.
Western Blot	Preliminary experiments gave an approx 28kDa band in Human Spleen, Tonsil and Peripheral Blood Mononucleocyte lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band
Immunohistochemistry	In paraffin embedded Human Tonsil shows staining of distinct parts of the nucleus in lymphoid cells. Recommended concentration, 3-6µg/ml.
Reference(s)	Goila-Gaur R, Khan MA, Miyagi E, Kao S, Strebel K. Targeting APOBEC3A to the viral nucleoprotein complex confers antiviral activity. Retrovirology. 2007 Aug 29;4