Goat anti-MARCH-I Antibody

Item#:

dAP-1350

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-MARCH-I Antibody
Principle Name	MARCH-I
Official Symbol	MARCH1
All Name and Symbols	MARCH1; membrane-associated ring finger (C3HC4) 1; DKFZp564M1682; FLJ20668; MARCH-I; RNF171; membrane-associated RING-CH protein I
Accession Number(s)	NP_060393.1
Peptide Sequence	QSRLSVCPSTQD, is from internal region
Human Gene ID(s)	55016
Non-Human GeneID(s)	72925 (mouse); 361135 (rat);
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA
Tested Species
Peptide ELISA	antibody detection limit dilution 1 to 32000.
Western Blot	Preliminary experiments in Human Colon, Ileum, Lung and Lymph Node lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results bee
Reference(s)	Matsuki Y, Ohmura-Hoshino M, Goto E, Aoki M, Mito-Yoshida M, Uematsu M, Hasegawa T, Koseki H, Ohara O, Nakayama M, Toyooka K, Matsuoka K, Hotta H, Yamamoto A, Ishido S. Novel regulation of MHC class II function in B cells. EMBO J. 2007 Feb 7;26(3)