Goat anti-RPE65 Antibody |
| Principle Name | RPE65 |
| Official Symbol | RPE65 |
| All Name and Symbols | mRPE65; sRPE65; retinal pigment epithelium-specific protein 65kDa; LCA2; RP20; rd12; RBP-binding membrane protein; p63; retinal pigment epithelium-specific 61 kDa protein; retinal pigment epithelium-specific protein (65kD); retinitis pigmentosa 20 |
| Accession Number(s) | NP_000320.1 |
| Peptide Sequence | EVKKNARKAPQPE, is from internal region |
| Human Gene ID(s) | 6121 |
| Non-Human GeneID(s) | |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Rat |
| Peptide ELISA | antibody detection limit dilution 1 to 32000. |
| Western Blot | Approx 65kDa band observed in Rat Retina lysates (calculated MW of 60.9kDa according to Human NP_000320.1 and 61.0kDa according to Rat NP_446014.1). Recommended concentration |
| Reference(s) | Rolling F, Le Meur G, Stieger K, Smith AJ, Weber M, Deschamps JY, Nivard D, Mendes-Madeira A, Provost N, Pereon Y, Cherel Y, Ali RR, Hamel C, Moullier P, Rolling F. Gene therapeutic prospects in early onset of severe retinal dystrophy |
