Goat anti-Frizzled 7 Antibody

Item#:

dAP-1324

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-Frizzled 7 Antibody
Principle Name	Frizzled 7
Official Symbol	FZD7
All Name and Symbols	rizzled 7; frizzled homolog 7 (Drosophila); FZD7; FzE3; Frizzled, drosophila, homolog of, 7
Accession Number(s)	NP_003498.1
Peptide Sequence	RFYHRLSHSSKGET, is from C Terminus
Human Gene ID(s)	8324
Non-Human GeneID(s)	14369 (mouse);
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB, IHC
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 64000.
Western Blot	Approx 60kDa band observed in lysates of cell lines HEK293 and HepG2 (calculated MW of 63.6kDa according to NP_003498.1). Recommended concentration
Immunohistochemistry	Paraffin embedded Human Spleen. Recommended concentration
Reference(s)	Vincan E, Darcy PK, Farrelly CA, Faux MC, Brabletz T, Ramsay RG. Frizzled-7 dictates three-dimensional organization of colorectal cancer cell carcinoids. Oncogene. 2007 Apr 5;26(16)