Goat anti-EAT2 phospho (Y127) Antibody

Item#:

dAP-1310

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-EAT2 phospho (Y127) Antibody
Principle Name	EAT2 phospho (Y127)
Official Symbol	SH2D1B
All Name and Symbols	SH2D1B; SH2 domain containing 1B; EAT2; SH2 domain-containing molecule EAT2
Accession Number(s)	NP_444512.2
Peptide Sequence	NSNSDpYVDVLP, is from internal region
Human Gene ID(s)	117157
Non-Human GeneID(s)
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Subsequently passed through a column with the non-phosphorylated version of the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 128000.
Western Blot	Approx 14kDa band observed in lysates of cell line A431 (calculated MW of 16.6kDa according to NP_002643.1). Recommended concentration
Reference(s)	Morra, M.; Lu, J.; Poy, F.; Martin, M.; Sayos, J.; Calpe, S.; Gullo, C.; Howie, D.; Rietdijk, S.; Thompson, A.; Coyle, A. J.; Denny, C.; Yaffe, M. B.; Engel, P.; Eck, M. J.; Terhorst, C. Structural basis for the interaction of the free SH2 domain EAT-2 with SLAM receptors in hematopoietic cells. EMBO J. 20