Goat anti-ABCB5 Antibody |
| Principle Name | ABCB5 |
| Official Symbol | ABCB5 |
| All Name and Symbols | ATP-binding cassette, sub-family B (MDR/TAP), member 5 ; ABCB5alpha; ABCB5beta; EST422562; ATP-binding cassette, sub-family B, member 5N |
| Accession Number(s) | NP_848654.3; NP_001157413.1 |
| Peptide Sequence | QTQHRNTSKKAQ , is from internal region |
| Human Gene ID(s) | 340273 |
| Non-Human GeneID(s) | |
| Important Comments | This antibody is expected to recognise both reported isoforms (NP_848654.3; NP_001157413.1) |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, FC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 32000. |
| Western Blot | Approx 80kDa band observed in lysates of cell line A431(calculated MW of 89.8kDa according to NP_848654.3). Recommended concentration |
| Reference(s) | Frank NY, Margaryan A, Huang Y, Schatton T, Waaga-Gasser AM, Gasser M, Sayegh MH, Sadee W, Frank MH. ABCB5-mediated doxorubicin transport and chemoresistance in human malignant melanoma. Cancer Res. 2005 May 15;65(10) |
