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Goat anti-PPP2R3A Antibody
Item#:
dAP-1289
Size Price Quantity
50.0 ug $310.00
Goat anti-PPP2R3A Antibody
Principle Name PPP2R3A
Official Symbol PPP2R3A
All Name and Symbols PPP2R3A; protein phosphatase 2 (formerly 2A), regulatory subunit B'', alpha ; PPP2R3; PR130; PR72; PP2A, subunit B, B''-PR72/PR130; PP2A, subunit B, B72/B130 isoforms; PP2A, subunit B, R3 isoform; Serine/threonine protein phosphatase 2A, 72/130 kDa regul
Accession Number(s) NP_002709.2; NP_871626.1
Peptide Sequence QKDVENDGPEPSD, is from internal region
Human Gene ID(s) 5523
Non-Human GeneID(s)
Important Comments This antibody is expected to recognise both reported isoforms (NP_002709.2; NP_871626.1).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 16000.
Western Blot Preliminary experiments gave an approx 80kDa band in Human Skeletal Muscle lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size
Immunohistochemistry Paraffin embedded Human Brain (Cerebellum). Recommended concentration
Reference(s) Zhang S, Cagatay T, Amanai M, Zhang M, Kline J, Castrillon DH, Ashfaq R, Oz OK, Wharton KA Jr. Viable mice with compound mutations in the Wnt/Dvl pathway antagonists nkd1 and nkd2. Mol Cell Biol. 2007 Jun;27(12)

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