Goat anti-MEPCE Antibody |
| Principle Name | MEPCE |
| Official Symbol | MEPCE |
| All Name and Symbols | MEPCE; methylphosphate capping enzyme; BCDIN3; FLJ20257; bin3, bicoid-interacting 3; bin3, bicoid-interacting 3, homolog |
| Accession Number(s) | NP_062552.2 |
| Peptide Sequence | KQQRKFQYGNYCK, is from internal region |
| Human Gene ID(s) | 56257 |
| Non-Human GeneID(s) | |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IHC |
| Tested Species | Rat, Human |
| Peptide ELISA | antibody detection limit dilution 1 to 2000. |
| Western Blot | Approx. 75kDa band observed in Rat Testis lysates (calculated MW of 74.4kDa according to Human NP_062552.2 and 75.3kDa according to Rat XP_222048.4). In transfected HEK293 transiently expressing Human MEPCE a band of approx. 75kDa is observ |
| Immunohistochemistry | In paraffin embedded Human Testis shows staining of the spermatids. Recommended concentration, 5-10µg/ml. |
| Reference(s) | Jeronimo C, Forget D, Bouchard A, Li Q, Chua G, Poitras C, Therien C, Bergeron D, Bourassa S, Greenblatt J, Chabot B, Poirier GG, Hughes TR, Blanchette M, Price DH, Coulombe B. Systematic analysis of the protein interaction network for the human transcription machinery reveals the identity of the 7SK capping enzyme. Mol Cell. 2007 Jul 20;27(2) |
