Goat anti-DIAPH3 Antibody

Item#:

dAP-1274

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-DIAPH3 Antibody
Principle Name	DIAPH3
Official Symbol	DIAPH3
All Name and Symbols	DIAPH3; diaphanous homolog 3 (Drosophila); RP11-26P21.1; DKFZP434C0931; DKFZp686A13178; DRF3; FLJ34705; diap3; diaphanous homolog 3; diaphanous-related formin 3
Accession Number(s)	NP_001035982.1; NP_112194.2
Peptide Sequence	RTPMPKDVRQS, is from internal region (near the C terminus)
Human Gene ID(s)	81624
Non-Human GeneID(s)
Important Comments	This antibody is expected to recognise both reported isoforms (NP_001035982.1 and NP_112194.2).
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA
Tested Species
Peptide ELISA	antibody detection limit dilution 1 to 64000.
Western Blot	Preliminary experiments gave an approx 50kDa band in human bone marrow, liver and lymph node lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given
Reference(s)	Yang C, Czech L, Gerboth S, Kojima S, Scita G, Svitkina T. Novel roles of formin mDia2 in lamellipodia and filopodia formation in motile cells. PLoS Biol. 2007 Nov;5(11)