Goat anti-Arylsulfatase B Antibody

Item#:

dAP-1269

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-Arylsulfatase B Antibody
Principle Name	Arylsulfatase B
Official Symbol	ARSB
All Name and Symbols	ARSB; arylsulfatase B; ASB; G4S; MPS6; N-acetylgalactosamine-4-sulfatase
Accession Number(s)	NP_000037.2; NP_942002.1
Peptide Sequence	KLARGHTNGTKPLD, is from internal region
Human Gene ID(s)	411
Non-Human GeneID(s)
Important Comments	This antibody is expected to recognise both reported isoforms (NP_000037.2; NP_942002.1).
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB, IHC
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 64000.
Western Blot	Approx 45kDa band observed in Human Heart lysates (calculated MW of 46.0kDa according to NP_942002.1). Recommended concentration
Immunohistochemistry	In paraffin embedded Human Skeletal Muscle shows striated pattern staining of majority of muscular fibres. Recommended concentration, 5-10µg/ml.
Reference(s)	Bhattacharyya S, Look D, Tobacman JK. Increased arylsulfatase B activity in cystic fibrosis cells following correction of CFTR. Clin Chim Acta. 2007 May 1;380(1-2)