Goat anti-BAG5 (internal) Antibody

Item#:

dAP-1260

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-BAG5 (internal) Antibody
Principle Name	BAG5 (internal)
Official Symbol	BAG5
All Name and Symbols	BAG5; BCL2-associated athanogene 5; BAG-5; BAG-family molecular chaperone regulator-5
Accession Number(s)	NP_001015048.1; NP_001015049.1; NP_004864.1
Peptide Sequence	DGNRTDKNYIR, is from internal region
Human Gene ID(s)	9529
Non-Human GeneID(s)	70369 (mouse); 366734 (rat);
Important Comments	This antibody is expected to recognise reported isoforms a (NP_001015049.1) and b (NP_001015048.1, NP_004864.1).
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA
Tested Species
Peptide ELISA	antibody detection limit dilution 1 to 16000.
Western Blot	Preliminary experiments in Human Brain (Cerebellum, Hippocampus and Substantia nigra) lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - h
Reference(s)	Kalia SK, Lee S, Smith PD, Liu L, Crocker SJ, Thorarinsdottir TE, Glover JR, Fon EA, Park DS, Lozano AM. BAG5 inhibits parkin and enhances dopaminergic neuron degeneration. Neuron. 2004 Dec 16;44(6)