Goat anti-NKD2 Antibody |
| Principle Name | NKD2 |
| Official Symbol | NKD2 |
| All Name and Symbols | NKD2; naked cuticle homolog 2 (Drosophila); Dvl-binding protein NKD2; naked cuticle homolog 2; naked cuticle-2 |
| Accession Number(s) | NP_149111.1 |
| Peptide Sequence | HKRYRQKGREGHS, is from internal region |
| Human Gene ID(s) | 85409 |
| Non-Human GeneID(s) | 72293 (mouse); 308068 (rat); |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA |
| Tested Species | |
| Peptide ELISA | antibody detection limit dilution 1 to 32000. |
| Western Blot | Preliminary experiments gave bands at approx 80kDa, 38kDa and 26kDa in Mouse Brain lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calcu |
| Reference(s) | Zhang S, Cagatay T, Amanai M, Zhang M, Kline J, Castrillon DH, Ashfaq R, Oz OK, Wharton KA Jr. Viable mice with compound mutations in the Wnt/Dvl pathway antagonists nkd1 and nkd2. Mol Cell Biol. 2007 Jun;27(12) |