Goat anti-WISP1 Antibody |
| Principle Name | WISP1 |
| Official Symbol | WISP1 |
| All Name and Symbols | WISP1; WNT1 inducible signaling pathway protein 1; CCN4; WISP1c; WISP1i; WISP1tc; WNT1 induced secreted protein 1; Wnt-1 inducible signaling pathway protein 1; wnt-1 signaling pathway protein 1 |
| Accession Number(s) | NP_003873.1; NP_543028.1 |
| Peptide Sequence | ESYPDFSEIAN, is from C Terminus |
| Human Gene ID(s) | 8840 |
| Non-Human GeneID(s) | 22402 (mouse); 65154 (rat); |
| Important Comments | This antibody is expected to recognize both reported isoforms (NP_003873.1; NP_543028.1). |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 128000. |
| Western Blot | In transfected HEK293 transiently expressing WISP1 a band of approx. 35kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration |
| Reference(s) | Major MB, Camp ND, Berndt JD, Yi X, Goldenberg SJ, Hubbert C, Biechele TL, Gingras AC, Zheng N, Maccoss MJ, Angers S, Moon RT. Wilms tumor suppressor WTX negatively regulates WNT/beta-catenin signaling. Science. 2007 May 18;316(5827) |
