Goat anti-Proenkephalin Antibody

Item#:

dAP-1248

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-Proenkephalin Antibody
Principle Name	Proenkephalin
Official Symbol	PENK
All Name and Symbols	proenkephalin; PENK
Accession Number(s)	NP_006202.1
Peptide Sequence	RSHHQDGSDNEE, is from internal region
Human Gene ID(s)	5179
Non-Human GeneID(s)
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB, IHC
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 32000.
Western Blot	Approx 37kDa band observed in Human Adrenal Gland lysates (calculated MW of 30.8kDa according to NP_006202.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Normant and Loh, Endocrino
Immunohistochemistry	Frozen section of Human Hypothalamus shows staining of dense enkephalinergic axon plexus and scattered neuronal cell bodies (higher magnification inset) in the human infundibular nucleus. Recommended concentration
Reference(s)	Schiltz CA, Bremer QZ, Landry CF, Kelley AE. Food-associated cues alter forebrain functional connectivity as assessed with immediate early gene and proenkephalin expression. BMC Biol. 2007 Apr 26;5