Goat anti-CRYAB Antibody |
| Principle Name | CRYAB |
| Official Symbol | CRYAB |
| All Name and Symbols | CRYAB; crystallin, alpha B; CRYA2; CTPP2; HSPB5; heat-shock 20 kD like-protein |
| Accession Number(s) | NP_001876.1 |
| Peptide Sequence | RLEKDRFSVNLD, is from internal region |
| Human Gene ID(s) | 1410 |
| Non-Human GeneID(s) | 12955 (mouse); 25420 (rat); |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Mouse, Rat |
| Peptide ELISA | antibody detection limit dilution 1 to 128000. |
| Western Blot | Approx 22kDa band observed in Mouse and Rat Eye lysates (calculated MW of 20.1kDa according to Mouse NP_034094.1 and Rat NP_037067.1). Recommended concentration |
| Reference(s) | Ousman SS, Tomooka BH, van Noort JM, Wawrousek EF, O'conner K, Hafler DA, Sobel RA, Robinson WH, Steinman L. Protective and therapeutic role for alphaB-crystallin in autoimmune demyelination. Nature. 2007 Jun 13; [Epub ahead of print].PMID |