Goat anti-CHD7 Antibody |
| Principle Name | CHD7 |
| Official Symbol | CHD7 |
| All Name and Symbols | chromodomain helicase DNA binding protein 7; FLJ20357; FLJ20361; IS3; KIAA1416; Scoliosis, idiopathic 3 |
| Accession Number(s) | NP_060250.2 |
| Peptide Sequence | KDEIDEFANSPSE, is from internal region |
| Human Gene ID(s) | 55636 |
| Non-Human GeneID(s) | 320790 (mouse); 312974 (rat); |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 64000. |
| Western Blot | Preliminary experiments gave bands at approx 250kDa and 48kDa in Rat Spinal Cord lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculate |
| Immunohistochemistry | Paraffin embedded Human Brain (Cortex). Recommended concentration |
| Reference(s) | Gao X, Gordon D, Zhang D, Browne R, Helms C, Gillum J, Weber S, Devroy S, Swaney S, Dobbs M, Morcuende J, Sheffield V, Lovett M, Bowcock A, Herring J, Wise C. CHD7 gene polymorphisms are associated with susceptibility to idiopathic scoliosis. Am J Hum Genet. 2007 May;80(5) |
