Goat anti-WIPF1 Antibody |
Principle Name | WIPF1 |
Official Symbol | WIPF1 |
All Name and Symbols | WIPF1; WAS/WASL interacting protein family, member 1 ; MGC111041; PRPL-2; WASPIP; WIP; Wiskott-Aldrich syndrome protein interacting protein |
Accession Number(s) | NP_003378.3; NP_001070737.1 |
Peptide Sequence | KLARNESRSGSNRRE, is from C Terminus |
Human Gene ID(s) | 7456 |
Non-Human GeneID(s) | 215280 (mouse); 117538 (rat); |
Important Comments | Both variants represent identical proteins |
Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
Applications | Pep ELISA, WB |
Tested Species | Human |
Peptide ELISA | antibody detection limit dilution 1 to 64000. |
Western Blot | Approx. 55kDa band observed in Human Lymph Node, Spleen and Tonsil lysates (calculated MW of 51.3kDa according to NP_003378.3 and NP_001070737.1). In transfected HEK293 transiently expressing WIPF1 a band of approx. 55kDa is observed. This |
Reference(s) | Massarwa R, Carmon S, Shilo BZ, Schejter ED. WIP/WASp-based actin-polymerization machinery is essential for myoblast fusion in Drosophila. Dev Cell. 2007 Apr;12(4) |