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Goat anti-Insulysin / Insulinase Antibody
Item#:
dAP-1187
Size Price Quantity
50.0 ug $310.00
Goat anti-Insulysin / Insulinase Antibody
Principle Name Insulysin / Insulinase
Official Symbol IDE
All Name and Symbols insulinase; insulysin; insulin-degrading enzyme ; FLJ35968; INSULYSIN
Accession Number(s) NP_004960.2; NP_001159418.1; NP_001309722.1; NP_001309723.1; NP_001309724.1; NP_001309726.1
Peptide Sequence QQYNFDRDNTE, is from internal region
Human Gene ID(s) 3416
Non-Human GeneID(s)
Important Comments This antibody is expected to recognize all reported isoforms (NP_004960.2; NP_001159418.1; NP_001309722.1; NP_001309723.1; NP_001309724.1; NP_001309726.1). Reported variants represent identical protein
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 32000.
Western Blot Approx 110-120kDa band observed in lysates of cell line K562 (calculated MW of 118kDa according to NP_004960.1). In transfected HEK293 transiently expressing Insulysin a band of approx. 110kDa is observed. This band is not observed in the n
Immunohistochemistry In paraffin embedded Human Lung shows cytoplasm staining of alveolar macrophages. Recommended concentration, 3-6µg/ml.
Reference(s) Zhao Z, Xiang Z, Haroutunian V, Buxbaum JD, Stetka B, Pasinetti GM. Insulin degrading enzyme activity selectively decreases in the hippocampal formation of cases at high risk to develop Alzheimer's disease. Neurobiol Aging. 2007 Jun;28(6)

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