Goat anti-Tcfec (mouse) Antibody

Item#:

dAP-1185

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-Tcfec (mouse) Antibody
Principle Name	Tcfec (mouse)
Official Symbol	Tcfec
All Name and Symbols	Tcfec; transcription factor EC ; BB107417; MGC107474; TFEC
Accession Number(s)	NP_112475.1
Peptide Sequence	HITKQQTHPERN, is from internal region
Human Gene ID(s)
Non-Human GeneID(s)	21426 (mouse); 26296 (rat);
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, IHC
Tested Species	Mouse, Human
Peptide ELISA	antibody detection limit dilution 1 to 32000.
Western Blot	Preliminary experiments gave one band at approx 37kDa and much stronger bands at approx 70+28kDa in Mouse Testis lysates after 0.5 µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the s
Immunohistochemistry	In paraffin embedded Mouse Thymus shows nuclear staining in sporadic cells of the medula. Recommended concentration
Reference(s)	Davis IJ, Kim JJ, Ozsolak F, Widlund HR, Rozenblatt-Rosen O, Granter SR, Du J, Fletcher JA, Denny CT, Lessnick SL, Linehan WM, Kung AL, Fisher DE. Oncogenic MITF dysregulation in clear cell sarcoma