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Goat anti-ITCH / AIF4 Antibody


Item#:
dAP-1182
Size Price Quantity
50.0 ug $310.00
Goat anti-ITCH / AIF4 Antibody
Principle Name ITCH / AIF4
Official Symbol ITCH
All Name and Symbols ITCH; AIF4; itchy homolog E3 ubiquitin protein ligase (mouse) ; AIP4; NAPP1; dJ468O1.1 ; NFE2-associated polypeptide 1; atrophin-1 interacting protein 4; dJ468O1.1 (atrophin 1 interacting protein 4, AIP4); itchy homolog E3 ubiquitin protein ligase; ubiqui
Accession Number(s) NP_113671.3
Peptide Sequence EIKSHDLKPNGGN, is from internal region
Human Gene ID(s) 83737
Non-Human GeneID(s) 16396 (mouse); 311567 (rat);
Important Comments
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 16000.
Western Blot Approx 90kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 98.7kDa according to NP_113671.3). Recommended concentration
Reference(s) Di Marcotullio L, Ferretti E, Greco A, De Smaele E, Po A, Sico MA, Alimandi M, Giannini G, Maroder M, Screpanti I, Gulino A. Numb is a suppressor of Hedgehog signalling and targets Gli1 for Itch-dependent ubiquitination. Nat Cell Biol. 2006 Dec;8(12)

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