Goat anti-BHMT Antibody |
| Principle Name | BHMT |
| Official Symbol | BHMT |
| All Name and Symbols | BHMT; betaine-homocysteine methyltransferase |
| Accession Number(s) | NP_001704.1 |
| Peptide Sequence | EQQLKELFEKQK, is from C Terminus |
| Human Gene ID(s) | 635 |
| Non-Human GeneID(s) | 12116 (mouse); 81508 (rat); |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IHC |
| Tested Species | Human, Mouse, Rat |
| Peptide ELISA | antibody detection limit dilution 1 to 16000. |
| Western Blot | Approx 45kDa band observed in Human Liver, Mouse Liver and Rat Liver lysates (calculated MW of 44.9kDa according to human NP_001704.1, 45.0kDa according to mouse NP_057877.1 and rat NP_110477.1). In transfected HEK293 transiently expressin |
| Immunohistochemistry | In paraffin embedded Human Kidney shows strong cytoplasm staining in PCT cells. Recommended concentration, 2-4µg/ml. |
| Reference(s) | Collinsova M, Strakova J, Jiracek J, Garrow TA. Inhibition of betaine-homocysteine S-methyltransferase causes hyperhomocysteinemia in mice. J Nutr. 2006 Jun;136(6) |
