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Goat anti-Fascin 2 Antibody
Item#:
dAP-1167
Size Price Quantity
50.0 ug $310.00
Goat anti-Fascin 2 Antibody
Principle Name Fascin 2
Official Symbol FSCN2
All Name and Symbols fascin homolog 2, actin-bundling protein, retinal (Strongylocentrotus purpuratus); RFSN; RP30; fascin 2; retinal fascin;
Accession Number(s) NP_001070650.1; NP_036550.1
Peptide Sequence CHHRGSNQLDTNR, is from internal region
Human Gene ID(s) 25794
Non-Human GeneID(s) 238021 (mouse); 303741 (rat);
Important Comments This antibody is expected to recognize both reported isoforms (NP_001070650.1 and NP_036550.1).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, IHC
Tested Species Mouse
Peptide ELISA antibody detection limit dilution 1 to 64000.
Western Blot Preliminary experiments gave bands at approx 75kDa and 22kDa in Mouse Eye lysates after 0.05µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated si
Immunohistochemistry This product was successfully used on sections of mouse cochlea as descibed in Shin et al, J Neurosci. 2010 Jul 21;30(29)
Reference(s) Vignjevic D, Kojima S, Aratyn Y, Danciu O, Svitkina T, Borisy GG. Role of fascin in filopodial protrusion. J Biol. 2006 Sep 11;174(6)

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