Goat anti-GPR3 Antibody |
| Principle Name | GPR3 |
| Official Symbol | GPR3 |
| All Name and Symbols | GPR3; G protein-coupled receptor 3 ; ACCA; OTTHUMP00000043209; adenylate cyclase constitutive activator |
| Accession Number(s) | NP_005272.1 |
| Peptide Sequence | LQRHLLPASH, is from internal region |
| Human Gene ID(s) | 2827 |
| Non-Human GeneID(s) | 14748 (mouse); 266769 (rat); |
| Important Comments | This antibody is not expected to cross-react with GPR6 and GPR12. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 32000. |
| Western Blot | Approx 35kDa band observed in Human Brain (Hippocampus and Frontal Cortex) lysates (calculated MW of 35.0kDa according to NP_005272.1). Recommended concentration |
| Reference(s) | Tanaka S, Ishii K, Kasai K, Yoon SO, Saeki Y. Neural expression of G protein-coupled receptors GPR3, GPR6, and GPR12 up-regulates cyclic AMP levels and promotes neurite outgrowth. J Biol Chem. 2007 Apr;282(14) |