Goat anti-HTATSF1 Antibody

Item#:

dAP-1162

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-HTATSF1 Antibody
Principle Name	HTATSF1
Official Symbol	HTATSF1
All Name and Symbols	HTATSF1; HIV-1 Tat specific factor 1 ; RP1-196E23.2; TAT-SF1; dJ196E23.2; HIV TAT specific factor 1; cofactor required for Tat activation of HIV-1 transcription
Accession Number(s)	NP_055315.2
Peptide Sequence	QELYGDGKDGDTQTD, is from internal region (near the N Terminus)
Human Gene ID(s)	27336
Non-Human GeneID(s)	317612 (rat);
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 16000.
Western Blot	Approx 140kDa band observed in lysates of cell line Daudi (calculated MW of 85.9kDa according to NP_055315.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Zhou and Sharp, Science.
Reference(s)	Zhou M, Deng L, Lacoste V, Park HU, Pumfery A, Kashanchi F, Brady JN, Kumar A. Coordination of transcription factor phosphorylation and histone methylation by the P-TEFb kinase during human immunodeficiency virus type 1 transcription. J Virol. 2004 Dec;78(24)