Goat anti-APH1A Antibody |
| Principle Name | APH1A |
| Official Symbol | APH1A |
| All Name and Symbols | APH1A; anterior pharynx defective 1 homolog A (C. elegans); 6530402N02Rik; APH-1A; CGI-78 ; anterior pharynx defective 1 homolog A |
| Accession Number(s) | NP_001071096.1; NP_057106.2 |
| Peptide Sequence | HVTDRSDARLQYG, is from internal region |
| Human Gene ID(s) | 51107 |
| Non-Human GeneID(s) | |
| Important Comments | This antibody is expected to recognize isoform 1 (NP_001071096.1) and isoform 2 (NP_057106.2). |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 128000. |
| Western Blot | Approx 26kDa band observed in Human Brain (Cerebral Cortex) lysates (calculated MW of 26.8kDa according to NP_057106.2). Recommended concentration |
| Immunohistochemistry | Paraffin embedded Human Placenta and Kidney. Recommended concentration |
| Reference(s) | Spasic D, Raemaekers T, Dillen K, Declerck I, Baert V, Serneels L, Fullekrug J, Annaert W. Rer1p competes with APH-1 for binding to nicastrin and regulates gamma-secretase complex assembly in the early secretory pathway. J Cell Biol. 2007 Feb 26;176(5) |
