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Goat anti-Fumarase / FH Antibody
Item#:
dAP-1139
Size Price Quantity
50.0 ug $310.00
Goat anti-Fumarase / FH Antibody
Principle Name Fumarase / FH
Official Symbol FH
All Name and Symbols fumarate hydratase; HLRCC; LRCC; MCL; MCUL1; fumarase; leiomyomatosis and renal cell cancer; multiple hereditary cutaneous leiomyomata
Accession Number(s) NP_000134.2
Peptide Sequence HPNDHVNKSQSSND, is from internal region
Human Gene ID(s) 2271
Non-Human GeneID(s) 14194 (mouse); 24368 (rat);
Important Comments
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB, IHC, EIA
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 64000.
Western Blot Approx 48kDa band observed in Human Kidney, Liver and Lung lysates (calculated MW of 54.6kDa according to NP_000134.2). The observed molecular weight corresponds to earlier findings with antibodies from different sources. In transfected HE
Immunohistochemistry Paraffin embedded Human Skeletal Muscle and Heart. Recommended concentration
Reference(s) Pollard PJ, Spencer-Dene B, Shukla D, Howarth K, Nye E, El-Bahrawy M, Deheragoda M, Joannou M, McDonald S, Martin A, Igarashi P, Varsani-Brown S, Rosewell I, Poulsom R, Maxwell P, Stamp GW, Tomlinson IP. Targeted inactivation of fh1 causes proliferative renal cyst development and activation of the hypoxia pathway. Cancer Cell. 2007 Apr;11(4)

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