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Goat anti-PIWI / HIWI Antibody
Item#:
dAP-1137
Size Price Quantity
50.0 ug $310.00
Goat anti-PIWI / HIWI Antibody
Principle Name PIWI / HIWI
Official Symbol PIWIL1
All Name and Symbols PIWIL1; HIWI; PIWI; piwi-like 1 (Drosophila); piwi (Drosophila)-like 1; homolog of Drosophila piwi; MIWI; piwi homolog; piwi-like 1
Accession Number(s) NP_004755.2; NP_001177900.1
Peptide Sequence SNRKDKYDAIKKY, is from internal region
Human Gene ID(s) 9271
Non-Human GeneID(s) 57749 (mouse);
Important Comments This antibody is expected to recognise both reported isoforms (NP_004755.2; NP_001177900.1).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 64000.
Western Blot Preliminary experiments showed a 100kDa band but also bands at approx 35kDa and 26-28kDa in Human Ovary, Placenta, Prostate and Testis lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the
Immunohistochemistry In paraffin embedded Human Testis shows textured cytoplasm staining in all germ cells. Recommended concentration, 4-6µg/ml.
Reference(s) Szakmary A, Cox DN, Wang Z, Lin H. Regulatory Relationship among piwi, pumilio, and bag-of-marbles in Drosophila Germline Stem Cell Self-Renewal and Differentiation. Curr Biol. 2005 Jan 26;15(2)

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