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Goat anti-MDA5 / Ifih1 (mouse) Antibody
Item#:
dAP-1136
Size Price Quantity
50.0 ug $310.00
Goat anti-MDA5 / Ifih1 (mouse) Antibody
Principle Name MDA5 / Ifih1 (mouse)
Official Symbol Ifih1
All Name and Symbols Ifih1; interferon induced with helicase C domain 1; 9130009C22Rik; Helicard; Hlcd; MDA-5; MDA5; MGC90959; melanoma differentiation associated protein-5
Accession Number(s) NP_082111.1
Peptide Sequence KQKLRNNDLKKQN, is from internal region
Human Gene ID(s)
Non-Human GeneID(s) 71586 (mouse);
Important Comments
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA
Tested Species
Peptide ELISA antibody detection limit dilution 1 to 16000.
Western Blot Preliminary experiments gave an approx 80kDa band in Mouse Brain lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 116kDa
Reference(s) Kato H, Takeuchi O, Sato S, Yoneyama M, Yamamoto M, Matsui K, Uematsu S, Jung A, Kawai T, Ishii KJ, Yamaguchi O, Otsu K, Tsujimura T, Koh CS, Reis e Sousa C, Matsuura Y, Fujita T, Akira S. Differential roles of MDA5 and RIG-I helicases in the recognition of RNA viruses. Nature. 2006 May 4;441(7089)

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