Goat anti-Collagen, type VII Antibody

Item#:

dAP-1111

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-Collagen, type VII Antibody
Principle Name	Collagen, type VII
Official Symbol	COL7A1
All Name and Symbols	Collagen, type VII; collagen, type VII, alpha 1 (epidermolysis bullosa, dystrophic, dominant and recessive); COL7A1; HGNC
Accession Number(s)	NP_000085.1
Peptide Sequence	TVQYSDDPRTEF, is from internal region
Human Gene ID(s)	1294
Non-Human GeneID(s)	12836 (mouse);
Important Comments	This product does not work on cryostat sections of Normal Human Skin.
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB
Tested Species	Rat
Peptide ELISA	antibody detection limit dilution 1 to 32000.
Western Blot	Approx 250kDa band observed in Rat Testis lysates (calculated MW of 159kDa according to Rat NP_001100328.1). Recommended concentration
Reference(s)	Ortiz-Urda S, Garcia J, Green CL, Chen L, Lin Q, Veitch DP, Sakai LY, Lee H, Marinkovich MP, Khavari PA. Type VII collagen is required for Ras-driven human epidermal tumorigenesis. Science. 2005 Mar 18;307(5716)