Goat anti-PODXL Antibody |
| Principle Name | PODXL |
| Official Symbol | PODXL |
| All Name and Symbols | PODXL; podocalyxin-like; Gp200; MGC138240; PCLP |
| Accession Number(s) | NP_001018121.1 ; NP_005388.2 |
| Peptide Sequence | DNLTKDDLDEEEDTH, is from C Terminus |
| Human Gene ID(s) | 5420 |
| Non-Human GeneID(s) | 27205 (mouse); 192181 (rat); |
| Important Comments | This antibody is expected to recognise both reported isoforms (NP_001018121.1 and NP_005388.2). |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human, Mouse, Rat |
| Peptide ELISA | antibody detection limit dilution 1 to 8000. |
| Western Blot | Approx 160kDa band observed in Human, Mouse and Rat Kidney lysates (calculated MW of 58.6kDa according to NP_001018121.1 and 55.4kDa according to NP_005388.2). The observed molecular weight corresponds to earlier findings in literature wit |
| Reference(s) | Nielsen JS, Graves ML, Chelliah S, Vogl AW, Roskelley CD, McNagny KM. The CD34-Related Molecule Podocalyxin Is a Potent Inducer of Microvillus Formation. PLoS ONE. 2007 Feb 21;2 |
