Goat anti-STAG3 (mouse) Antibody

Item#:

dAP-1101

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-STAG3 (mouse) Antibody
Principle Name	STAG3 (mouse)
Official Symbol	STAG3
All Name and Symbols	STAG3 ; stromal antigen 3 ; SCC3 homolog 3; cohesin subunit SA-3; stromalin 3
Accession Number(s)	NP_058660.2
Peptide Sequence	KHYNKFYEDYGD, is from internal region
Human Gene ID(s)	10734
Non-Human GeneID(s)	50878 (mouse); 114522 (rat);
Important Comments	The immunizing peptide was designed based on the Mouse protein sequence with one residue of a diference from the human sequence.
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA
Tested Species
Peptide ELISA	antibody detection limit dilution 1 to 32000.
Western Blot	Preliminary experiments gave an approx 30kDa band in Human Testis lysates after 0.5µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 141k
Reference(s)	Storre J, Schafer A, Reichert N, Barbero JL, Hauser S, Eilers M, Gaubatz S. Silencing of the meiotic genes SMC1beta and STAG3 in somatic cells by E2F6. J Biol Chem. 2005 Dec 16;280(50)