Goat anti-TIA1 Antibody |
| Principle Name | TIA1 |
| Official Symbol | TIA1 |
| All Name and Symbols | TIA1; TIA1 cytotoxic granule-associated RNA binding protein ; TIA1 cytotoxic granule-associated RNA-binding protein; TIA1 protein; cytotoxic granule-associated RNA-binding protein; p40-TIA-1 (containing p15-TIA-1) |
| Accession Number(s) | NP_071320.1; NP_071505.1 |
| Peptide Sequence | PKSTYESNTKQ, is from internal region |
| Human Gene ID(s) | 7072 |
| Non-Human GeneID(s) | 21841 (mouse); 312510 (rat); |
| Important Comments | This antibody is expected to recognise both reported isoforms (NP_071320.1 and NP_071505.1). |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IF, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 128000. |
| Western Blot | Approx 40kDa band observed in lysates of cell line Jurkat (calculated MW of 41.8kDa according to NP_071320.1). Recommended concentration |
| Immunohistochemistry | Paraffin embedded Human Thyroid. Recommended concentration |
| Reference(s) | Yang F, Peng Y, Murray EL, Otsuka Y, Kedersha N, Schoenberg DR. Polysome-bound endonuclease PMR1 is targeted to stress granules via stress-specific binding to TIA-1. Mol Cell Biol. 2006 Dec;26(23) |
