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Goat anti-SORBS1/ponsin (internal region) Antibody
Item#:
dAP-1087
Size Price Quantity
50.0 ug $310.00
Goat anti-SORBS1/ponsin (internal region) Antibody
Principle Name SORBS1/ponsin (internal region)
Official Symbol SORBS1
All Name and Symbols SORBS1; sorbin and SH3 domain containing 1; CAP; DKFZp451C066; DKFZp586P1422; FLAF2; FLJ12406; KIAA1296; R85FL; SH3D5; SH3P12; SORB1 ; Fas-ligand associated factor 2; SH3-domain protein 5; SH3-domain protein 5 (ponsin); c-Cbl associated protein; ponsin
Accession Number(s) NP_006425.2; NP_056200.1; NP_001030126.1; NP_001030127.1; NP_001030128.1; NP_079267.1; NP_001030129.1
Peptide Sequence SERNDWEPPDKKVD, is from internal region
Human Gene ID(s) 10580
Non-Human GeneID(s) 20411 (mouse);
Important Comments This antibody is expected to recognise all reported isoforms (NP_006425.2 and NP_056200.1 and NP_001030126.1 and NP_001030127.1 and NP_001030128.1 and NP_079267.1 and NP_001030129.1)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA
Tested Species
Peptide ELISA antibody detection limit dilution 1 to 32000.
Western Blot Preliminary experiments gave an approx 65kDa band in Human Adipose lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 76.
Reference(s) Baumann CA, Ribon V, Kanzaki M, Thurmond DC, Mora S, Shigematsu S, Bickel PE, Pessin JE, Saltiel AR. CAP defines a second signalling pathway required for insulin-stimulated glucose transport. Nature. 2000 Sep 14;407(6801)

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