Goat anti-APOC1 Antibody |
Principle Name | APOC1 |
Official Symbol | APOC1 |
All Name and Symbols | APOC1; apolipoprotein C-I |
Accession Number(s) | NP_001636.1 |
Peptide Sequence | ETFQKVKEKLKIDS, is from C Terminus |
Human Gene ID(s) | 341 |
Non-Human GeneID(s) | |
Important Comments | |
Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
Applications | Pep ELISA, IHC |
Tested Species | Human |
Peptide ELISA | antibody detection limit dilution 1 to 8000. |
Western Blot | Preliminary experiments gave bands at 30kDa and 37kDa band in Human Liver lysates after 0.5 µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated siz |
Immunohistochemistry | In paraffin embedded Human Liver shows spotty staining in the cytoplasm of hepatocytes. Recommended concentration, 3-5µg/ml. |
Reference(s) | Berbee JF, van der Hoogt CC, Sundararaman D, Havekes LM, Rensen PC. Severe hypertriglyceridemia in human APOC1 transgenic mice is caused by apoC-I-induced inhibition of LPL. J Lipid Res. 2005 Feb;46(2) |