Goat anti-DKC1 Antibody |
| Principle Name | DKC1 |
| Official Symbol | DKC1 |
| All Name and Symbols | DKC1; dyskeratosis congenita 1, dyskerin ; DKC; NAP57; NOLA4; XAP101; |
| Accession Number(s) | NP_001354.1 |
| Peptide Sequence | KRKRESESESDETPP, is from internal region |
| Human Gene ID(s) | 1736 |
| Non-Human GeneID(s) | 245474 (mouse); |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 128000. |
| Western Blot | Preliminary experiments gave an approx 70-75kDa band in Human Bone Marrow, Duodenum and Skin lysates after 0.01µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given |
| Immunohistochemistry | In paraffin embedded Human Spleen shows strong nuclear staining of select splenocytes. Recommended concentration, 5-10µg/ml. |
| Reference(s) | Yoon A, Peng G, Brandenburger Y, Zollo O, Xu W, Rego E, Ruggero D. Impaired control of IRES-mediated translation in X-linked dyskeratosis congenita. Science. 2006 May 12;312(5775) |
