Goat anti-NANOG (C Terminus) Antibody

Item#:

dAP-1073

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-NANOG (C Terminus) Antibody
Principle Name	NANOG (C Terminus)
Official Symbol	NANOG
All Name and Symbols	NANOG; Nanog homeobox; homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48
Accession Number(s)	NP_079141.2
Peptide Sequence	DLFLNYSMNMQPED, is from C Terminus
Human Gene ID(s)	79923
Non-Human GeneID(s)
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB, IHC, EIA
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 128000.
Western Blot	Approx 35kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 34.6kDa according to NP_079141.1). Recommended concentration
Immunohistochemistry	In paraffin embedded Human Testis shows nuclear staining in spermatogonia. Recommended concentration, 1-2µg/ml.
Reference(s)	Yasuda SY, Tsuneyoshi N, Sumi T, Hasegawa K, Tada T, Nakatsuji N, Suemori H. NANOG maintains self-renewal of primate ES cells in the absence of a feeder layer. Genes Cells. 2006 Sep;11(9)