Goat anti-PIAS2 Antibody |
| Principle Name | PIAS2 |
| Official Symbol | PIAS2 |
| All Name and Symbols | PIAS2; protein inhibitor of activated STAT, 2 ; MGC102682; MIZ1; PIASX-ALPHA; PIASX-BETA; SIZ2; miz ; Msx-interacting-zinc finger; protein inhibitor of activated STAT X; ARIP3 |
| Accession Number(s) | NP_775298.1; NP_004662.2 |
| Peptide Sequence | KEAMKVSSQPCTKIE, is from internal region |
| Human Gene ID(s) | 9063 |
| Non-Human GeneID(s) | 17344 (mouse); |
| Important Comments | This antibody is expected to recognise both reported isoforms (NP_775298.1 and NP_004662.2). No cross-reactivity expected with PIAS1, 3 and 4. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 8000. |
| Western Blot | Preliminary experiments gave an approx 90kDa band in lysates of cell line A431after 0.5µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of |
| Immunohistochemistry | In paraffin embedded Human Testis shows nuclear and extra-nuclear staining in spermatids. Recommended concentration, 3-5µg/ml. |
| Reference(s) | van den Akker E, Ano S, Shih HM, Wang LC, Pironin M, Palvimo JJ, Kotaja N, Kirsh O, Dejean A, Ghysdael J. FLI-1 functionally interacts with PIASxalpha, a member of the PIAS E3 SUMO ligase family. J Biol Chem. 2005 Nov 11;280(45) |
