Goat anti-S3-12 / KIAA1881 Antibody

Item#:

dAP-1062

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-S3-12 / KIAA1881 Antibody
Principle Name	S3-12 / KIAA1881
Official Symbol	KIAA1881
All Name and Symbols	KIAA1881; S3-12; plasma membrane associated protein, S3-12; hCG1646516
Accession Number(s)	NP_001073869.1
Peptide Sequence	RTTDNGGEQTALSPQE, is from internal region
Human Gene ID(s)	729359
Non-Human GeneID(s)
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, IHC
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 2000.
Western Blot	Preliminary experiments gave bands at approx 45kDa and 50kDa in Human Adipose lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated
Immunohistochemistry	In paraffin embedded Human Pancreas shows membranous and cytoplasm staining in adipocytes. Recommended concentration, 3-6µg/ml.
Reference(s)	Wolins NE, Brasaemle DL, Bickel PE. A proposed model of fat packaging by exchangeable lipid droplet proteins. FEBS Lett. 2006 Oct 9;580(23)