Goat anti-OGT Antibody |
| Principle Name | OGT |
| Official Symbol | OGT |
| All Name and Symbols | OGT; O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine |
| Accession Number(s) | NP_858058.1; NP_858059.1 |
| Peptide Sequence | YEHPKDLKLSDGR, is from internal region |
| Human Gene ID(s) | 8473 |
| Non-Human GeneID(s) | 108155 (mouse); 26295 (rat); |
| Important Comments | This antibody is expected to recognise both reported isoforms (NP_858058.1 and NP_858059.1 |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IHC |
| Tested Species | Human, Rat |
| Peptide ELISA | antibody detection limit dilution 1 to 64000. |
| Western Blot | Approx 110kDa band observed in Pancreas lysates from Rat (calculated MW of 117kDa according to NP_858058.1. Recommended concentration |
| Immunohistochemistry | Paraffin embedded Human Brain (Cortex). Recommended concentration |
| Reference(s) | Majumdar G, Wright J, Markowitz P, Martinez-Hernandez A, Raghow R, Solomon SS. Insulin stimulates and diabetes inhibits O-linked N-acetylglucosamine transferase and O-glycosylation of Sp1. Diabetes. 2004 Dec;53(12) |
