Goat anti-P4HA1 Antibody |
| Principle Name | P4HA1 |
| Official Symbol | P4HA1 |
| All Name and Symbols | P4HA1; procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide I; 4-PH alpha-1; P4HA ; prolyl 4-hydroxylase, alpha I subunit |
| Accession Number(s) | NP_000908.2; NP_001017962.1 |
| Peptide Sequence | DFARKDEPDAFKE, is from internal region |
| Human Gene ID(s) | 5033 |
| Non-Human GeneID(s) | 18451 (mouse); 64475 (rat); |
| Important Comments | This antibody is expected to recognise both reported isoforms (NP_000908.2 and NP_001017962.1). |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 32000. |
| Western Blot | Approx 60kDa band observed in lysates of cell lines HEK293 and A431 (calculated MW of 61.0kDa according to NP_000908.2 and NP_001017962.1). Recommended concentration |
| Immunohistochemistry | Paraffin embedded Human Skin and Spleen. Recommended concentration |
| Reference(s) | Annunen P, Helaakoski T, Myllyharju J, Veijola J, Pihlajaniemi T, Kivirikko KI. Cloning of the human prolyl 4-hydroxylase alpha subunit isoform alpha(II) and characterization of the type II enzyme tetramer. The alpha(I) and alpha(II) subunits do not form a mixed alpha(I)alpha(II)beta2 tetramer. J Biol Chem. 1997 Jul 11;272(28) |
