Goat anti-IER5 Antibody |
| Principle Name | IER5 |
| Official Symbol | IER5 |
| All Name and Symbols | IER5; immediate early response 5; MGC102760; SBBI48 |
| Accession Number(s) | NP_057629.1 |
| Peptide Sequence | QPPSGGEDDDAEE, is from internal region |
| Human Gene ID(s) | 51278 |
| Non-Human GeneID(s) | |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 2000. |
| Western Blot | Customer's experiments gave a band at approx 45kDa in lysates of transfected H1299 overexpressing IER5 but not when transfected with empty vector (calculated MW of 33.7kDa according to NP_057629.1). Recommended concentration |
| Reference(s) | Cirelli C, Tononi G. Gene expression in the brain across the sleep-waking cycle. Brain Res. 2000 Dec 8;885(2) |
