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Goat anti-C14orf68 (aa242-254) Antibody
Item#:
dAP-1005
Size Price Quantity
50.0 ug $310.00
Goat anti-C14orf68 (aa242-254) Antibody
Principle Name C14orf68 (aa242-254)
Official Symbol C14orf68
All Name and Symbols C14orf68; chromosome 14 open reading frame 68 ; HDMCP; HMFN1655 ; hepatocellular carcinoma-downregulated mitochondrial carrier protein
Accession Number(s) NP_997000.2
Peptide Sequence KSRLQADGQGQRR, is from internal region
Human Gene ID(s) 283600
Non-Human GeneID(s)
Important Comments
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 32000.
Western Blot Preliminary experiments gave an approx 22kDa band in Human Liver lysates after 0.5µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 33.4k
Immunohistochemistry In paraffin embedded Human Liver shows staining of nucleoli in select hepatocytes. Recommended concentration, 2.5µg/ml.
Reference(s) Tan MG, Ooi LL, Aw SE, Hui KM. Cloning and identification of hepatocellular carcinoma down-regulated mitochondrial carrier protein, a novel liver-specific uncoupling protein. J Biol Chem. 2004 Oct 22;279(43)

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