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Goat anti-TCF3 / ITF1 Antibody
Item#:
dAP-1001
Size Price Quantity
50.0 ug $310.00
Goat anti-TCF3 / ITF1 Antibody
Principle Name TCF3 / ITF1
Official Symbol TCF3
All Name and Symbols TCF3; ITF1; transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47); E2A; MGC129647; MGC129648 ; E2A immunoglobulin enhancer-binding factor E12/E47; immunoglobulin transcription factor 1; kappa-E2-binding factor; transcription factor
Accession Number(s) NP_003191.1
Peptide Sequence KAPRARTSPDEDED, is from internal region
Human Gene ID(s) 6929
Non-Human GeneID(s)
Important Comments This antibody is expected to recognize reported isoform E12 (NP_003191.1) only.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 128000.
Western Blot Approx 75kDa band observed in lysates of cell line Daudi (calculated MW of 67.6kDa according to NP_003191.1). Recommended concentration
Immunohistochemistry In paraffin embedded Human Kidney shows strong nuclear staining in DCT cells. Recommended concentration, 3-5µg/ml.
Reference(s) Greenbaum S, Lazorchak AS, Zhuang Y. Differential functions for the transcription factor E2A in positive and negative gene regulation in pre-B lymphocytes. J Biol Chem. 2004 Oct 22;279(43)

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