Goat anti-TCF2 / VHNF1 Antibody

Item#:

dAP-1000

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-TCF2 / VHNF1 Antibody
Principle Name	TCF2 / VHNF1
Official Symbol	HNF1B
All Name and Symbols	TCF2; VHNF1; transcription factor 2, hepatic LF-B3 variant hepatic nuclear factor; FJHN; HNF1B; HNF1beta; HNF2; LFB3; MODY5; transcription factor 2
Accession Number(s)	NP_000449.1; NP_001159395.1
Peptide Sequence	QAYDRQKNPSKEER, is from internal region
Human Gene ID(s)	6928
Non-Human GeneID(s)	21410 (mouse); 25640 (rat);
Important Comments	This antibody is expected to recognize both reported isoforms (NP_000449.1; NP_001159395.1). This product may cross-react with HNF1A.
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB, ICC
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 64000.
Western Blot	Approx 60kDa band observed in Human Liver lysates (calculated MW of 61.3kDa according to NP_000449.1). Recommended concentration
Reference(s)	Terasawa K, Toyota M, Sagae S, Ogi K, Suzuki H, Sonoda T, Akino K, Maruyama R, Nishikawa N, Imai K, Shinomura Y, Saito T, Tokino T. Epigenetic inactivation of TCF2 in ovarian cancer and various cancer cell lines. Br J Cancer. 2006 Mar 27;94(6)