Goat anti-PRPF31 Antibody

Item#:

dAP-0994

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-PRPF31 Antibody
Principle Name	PRPF31
Official Symbol	PRPF31
All Name and Symbols	PRPF31; PRP31 pre-mRNA processing factor 31 homolog (S. cerevisiae); DKFZp566J153; NY-BR-99; PRP31; RP11 ; PRP31 pre-mRNA processing factor 31 homolog (yeast); pre-mRNA processing factor 31 homolog; pre-mRNA processing factor 31 homolog (yeast)
Accession Number(s)	NP_056444.3
Peptide Sequence	KELGNSLDKCKNNEN, is from internal region
Human Gene ID(s)	26121
Non-Human GeneID(s)	68988 (mouse);
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 128000.
Western Blot	Approx 60kDa band observed in lysates of cell lines A431 and HeLa (calculated MW of 55.4kDa according to NP_056444.2). Recommended concentration
Reference(s)	Yuan L, Kawada M, Havlioglu N, Tang H, Wu JY. Mutations in PRPF31 inhibit pre-mRNA splicing of rhodopsin gene and cause apoptosis of retinal cells. J Neurosci. 2005 Jan 19;25(3)